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What is Nigella Sativa?
(Jintan Hitam)

http://www.nigella-sativa-seeds.com/

Photo Credit: Wikipedia

 

Nigella Sativa is a flowering plant, native to southwest Asia that grows to 20-30 cm tall with linear leaves. The flowers are delicate, and usually colored pale blue and white, with 5-10 petals. The fruit is a large and inflated capsule composed of 3-7 united follicles, each containing numerous seeds. The seed is used as a spice.

There is a huge understanding about the names of this spice. Nigella Sativa seed is also known as Kalonji (Hindi), Kezah (Hebrew), Habbat-el-barakah (literally seeds of blessing Arabic) or Siyah Daneh (Persian). In English, it is called Fennel Flower, Black Caraway, Nutmeg Flower or Roman Coriander. Other names used, sometimes misleadingly, are Onion Seed and Black Sesame. Frequently the seeds are referred to as Black Cumin; this is, however, also used for a different spice, Bunium Persicum. It is also sometimes just referred to as Nigella or Black Seed. An old English name gith is now used for the corn cockle.

Nigella Sativa belongs to the Plant family of Ranunculaceae (buttercup family). Nigella seeds have little odour, but when ground or chewed they develop a vaguely oregano-like scent. The taste is aromatic and slightly bitter; I have seen it called “pungent” and “smoky” and even compared to black pepper, but I cannot agree with that comparison. There is, however, some pungency in unripe or not yet dried seeds.

Nigella Sativa originated probably from Western Asia. Although nigella is not mentioned in the common Bible translations, there is good evidence that an obscure plant name mentioned in the Old Testament means nigella; if true, this would indicate that nigella is cultivated since far more than two millennia. Today, the plant is cultivated from Egypt to India.

The seeds contain numerous esters of structurally unusual unsaturated fatty acids with terpene alcohols (7%); furthermore, traces of alkaloids are found which belong to two different types: isochinoline alkaloids are represented by nigellimin and nigellimin-N-oxide, and pyrazol alkaloids include nigellidin and nigellicin.

In the essential oil (avr. 0.5%, max. 1.5%), thymoquinone was identified as the main component (up to 50%) besides p-cymene (40%), a-pinene (up to 15%), dithymoquinone and thymohydroquinone. Other terpene derivatives were found only in trace amounts: Carvacrol, carvone, limonene, 4-terpineol, citronellol. Furthermore, the essential oil contains significant (10%) amounts of fatty acid ethyl esters. On storage, thymoquinone yields dithymoquinonene and higher oligocondensation products (nigellone).

The seeds also contain a fatty oil rich in unsaturated fatty acids, mainly linoleic acid (50 – 60%), oleic acid (20%), eicodadienoic acid (3%) and dihomolinoleic acid (10%) which is characteristic for the genus. Saturated fatty acids (palmitic, stearic acid) amount to about 30% or less. Commercial nigella oil (“Black Seed Oil”, “Black Cumin Oil”) may also contain parts of the essential oil, mostly thymoquinone, by which it acquires an aromatic flavour.

 

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http://www.nigella-sativa-seeds.com/scientific-analysis.html

Scientific Analysis of Nigella Sativa

Nigella Sativa contains several ingredients with potential value. The following values reflect the composition of Nagilla Sativa in terms of its active, nutrient components, and any other significant ingredients.



Fundamental Oil Composition (1.4%) Nigella Sativa
Carvone 21.1%
Alfa-Pinene 7.4%
Sabinene 5.5%
Beta-Pinene 7.7%
P-cymene 46.8%
Others 11.5%
Fatty Acids Nigella Sativa
Myristic Acid (C14:0) 0.5%
Palmitic Acid (C16:0) 13.7%
Palmitoleic Acid (C16:1) 0.1%
Stearic Acid (C18:0) 2.6%
Oleic Acid (C18:1) 23.7%
Linoleic Acid (C18:2)(Omega-6) 57.9%
Linolenic Acid (18:3n-3) (Omega-3) 0.2%
Arachidic Acid (C20:0) 1.3%
Saturated & Unsaturated Fatty Acids Nigella Sativa
Saturated Acid 18.1%
Monounsaturated Acids 23.8%
Polyunsaturated Acids 58.1%
Nutritional Value Nigella Sativa
Protein 208 ug/g
Thiamin 15ug/g
Riboflavin 1 ug/g
Pyridoxine 5ug/g
Niacin 57 ug/g
Folacin 610 IU/g
Calcium 1.859 mg/g
Iron 105 ug/g
Copper 18 ug/g
Zinc 60 ug/g
Phosphorus 5.265 mg/g
Nutritional Composition Nigella Sativa
protein 21%
carbohydrates 35%
fats 35-38%

 

Discover Miraculous Medicinal Uses of Nigella Sativa

The seeds of Nigella Sativa are beneficial for the digestive system, soothing stomach pains and spasms and easing wind, bloating and colic. The ripe seed is anthelmintic, carminative, diaphoretic, digestive, diuretic, emmenagogue, galactogogue, laxative and stimulant. An infusion is used in the treatment of digestive and menstrual disorders, insufficient lactation and bronchial complaints. The seeds are used in India to increase the flow of milk in nursing mothers and can be used to treat intestinal worms, especially in children. Externally, the seed is ground into a powder, mixed with sesame oil and used to treat abscesses, haemorrhoids and orchitis. The powdered seed been used to remove lice from the hair. The Black Cumin (Nigella sativa) Seed is also a source of calcium, iron, sodium, and potassium. Required only in small amounts by the body, these elements' main function is to act as essential cofactors in various enzyme functions.

Other Properties

Stimulant, aromatic, carminative, digestive, diuretic, emmenagogue, excitant, galactatagogue, purgative, resolvent, stimulant, stomachic, sudorific, tonic, anthelmintic, diaphoretic, parasiticide, and vermifuge. The seed contains about 1.5% essential oil. It is placed amongst clothes etc to repel moths. The seeds can also be put in muslin bags and hung near a fire when they will fill the room with their delicious scent. They need to be changed about every three weeks. The seed contains 35% of a fatty oil. Black seed contains Arginine which is essential for infant growth.

 

Botanical Information

Family: Ranunculaceae

Botanical Name: Nigella sativa L.

Physical Characteristics: The seeds are tiny (1-3 mm long), black, three sided and look a bit like pieces of flint under a microscope having a strong, spicy, peppery taste.

Common Names: Ajenuz, Black Caraway, Black Cumin, Black Seed, Corekotu, Charnushka, Faux Cumin, Fennel Flower, Fitch, Habba Soda, Habat-al-Baraka, Kaladuru, Kolonji, Kalonji, Nutmeg Flower, Roman Coriander, Schwarzkummel.

Range: N. Africa to Ethiopia and W. Asia

Habitats: Cultivated Beds. Easily grown in any good garden soil, preferring a sunny position. Prefers a light soil in a warm position. This species is often cultivated, especially in western Asia and India, for its edible seed. The seed is aromatic with a nutmeg scent. A greedy plant, inhibiting the growth of nearby plants, especially legumes.

Edible Part: Seed

Edible Use: Condiment

Energy and Flavors: Hot energy, Spicy flavor

Systems Affected: Lungs, Stomach, Spleen

Biochemical Constituents: Alanine, arginine, ascorbic-acid, asparagine, campesterol, carvone, cymene, cystine, dehydroascorbic-acid, eicosadienoic-acid, glucose, glutamic-acid, glycine, iron, isoleucine, leucine, d-limonene, linoleic-acid, linolenic-acid, lipase, lysine, methionine, myristic-acid, nigellin, nigellone, oleic-acid, palmitic-acid, phenylalanine, phytosterols, potassium, beta-sitosterol, alpha-spinasterol, stearic-acid, stigmasterol, tannin, threonine, thymohydroquinone, thymoquinone, tryptophan, tyrosine

 

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Clinical Data on Black Seed Herb


Mei 28, 2008 — Bin Muhsin

http://formulacinta.wordpress.com/category/keajaiban-jintan-hitam/

Clinical Data on Black Seed Herb

Black seed is used for treating gastrointestinal conditions including gas, colic, diarrhea, dysentery, constipation and haemorrhoids. It is also used for respiratory conditions, including asthma, allergies, cough, bronchitis, emphysema, flu and congestion. Additionally, it is used as an antihypertensive, immunoprotectant, anticancer agent, and vermifuge. It is used orally for women’s health: including as a contraceptive, for stimulation of menstruation, and increasing milk flow.

In allergic conditions, black seed may have antihistamine effects. Black seed is thought to have immunoprotectant effects. Preliminary evidence suggests it may help minimize chemotherapy-induced decreases in hemoglobin and leukocyte counts. Black seed may be beneficial as an anticancer agent. According to preliminary studies, black seed may inhibit stomach tumors, carcinoma, and Ehrlich ascites carcinoma.

Black seed is reported to have been used for over 2000 years. Recordings mention it as far back as 1400 years. Black seed was found in the tomb of King Tutankhamen.

Clinical Studies – References – Buy Black Seed Oil now

Published Clinical Studies
Black Seed Herb

* The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production.
* Neuropharmacological activity of Nigella sativa L. extracts.
* Chemopreventive potential of volatile oil from black cumin (Nigella sativa L.) seeds against rat colon carcinogenesis.
* Nigella sativa (black cumin) ameliorates potassium bromate-induced early events of carcinogenesis: diminution of oxidative stress.
* Delayed apoptosis upon the treatment of Hep-2 cells with black seed.
* Effect of Nigella sativa (N. sativa L.) and oxidative stress on the survival pattern of MCF-7 breast cancer cells.
* Protective effect of Nigella sativa seeds against carbon tetrachloride-induced liver damage.
* Effect of Nigella sativa (black seed) on subjective feeling in patients with allergic diseases.

The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production.

Mahmood MS, Gilani AH, Khwaja A, Rashid A, Ashfaq MK.

Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi, Pakistan.

The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide (NO) production by murine macrophages was studied. Murine peritoneal macrophages were pre-incubated with the extract and then activated with Escherichia coli lipopolysaccharride. NO production was measured after 24 hours by spectrophotometry. The plant extract caused a dose-dependent decrease in NO production. Dialyzed preparation of the extract did not affect NO production. However, the boiled fraction of the extract resulted in a dose-dependent inhibition of NO apparently comparable to that of the whole extract. These results indicate that the aqueous extract of N. sativa seeds exhibits an inhibitory effect on nitric oxide production by murine macrophages and the active component(s) is/are non-protein in nature. In view of the fact that nitric oxide is a pro-inflammatory mediator, this study validates the traditional use of the Nigella sativa seeds for the treatment of rheumatism. Copyright 2003 John Wiley & Sons, Ltd.

PMID: 13680825 [PubMed - indexed for MEDLINE]

More Info – Clinical Studies – References – Buy Black Seed Oil now

Neuropharmacological activity of Nigella sativa L. extracts.

Al-Naggar TB, Gomez-Serranillos MP, Carretero ME, Villar AM.

Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.

Pharmacological studies have been conducted on the aqueous and methanol extracts of defatted Nigella sativa L. seeds to evaluate their effects on the central nervous system (CNS) and on analgesic activity. The observations suggest that the two extracts of Nigella sativa possesses a potent CNS and analgesic activity (depressant action especially in the case of the methanolic extract).

PMID: 12902052 [PubMed - indexed for MEDLINE]

More Info – Clinical Studies – References – Buy Black Seed Oil now

Chemopreventive potential of volatile oil from black cumin (Nigella sativa L.) seeds against rat colon carcinogenesis.

Salim EI, Fukushima S.

Laboratory of Experimental and Molecular Carcinogenesis, Department of Zoology, Faculty of Science, Tanta University, Tanta 31527-Egypt.

Chemopreventive effects of orally administered Nigella sativa oil on the induction and development of 1,2-dimethylhydrazine-induced aberrant crypt foci (ACF), putative preneoplastic lesions for colon cancer, were investigated in Fischer 344 rats. Starting at 6 wk of age, 45 male rats (groups 1-3) were subcutaneously injected with DMH once a week for 3 wk. Group 1 (15 rats) served as a carcinogen control group without N. sativa administration. Group 2 or 3 (15 rats each) were given the oil in the postinitiation stage or in the initiation stage, respectively. Animals of group 4 (11 rats) were injected with 0.9% saline and received N. sativa oil from the beginning until the termination. At sacrifice, 14 wk after the start, the total numbers of ACF as well as those with at least four crypts were significantly reduced in group 2 (P < 0.01). However, treatment with N. sativa oil in the initiation stage (group 3) did not exhibit significant inhibitory effects except on foci with only one aberrant crypt. Immunohistochemical analysis of 5-bromo-2?.-deoxyuridine labeling in colonic crypts revealed the N. sativa oil to have significant antiproliferative activity in both initiation and postinitiation stages and especially in the latter. Histological examination revealed no pathological changes in the liver, kidneys, spleen, or other organs of rats treated with N. sativa. In addition, biochemical parameters of blood and urine as well as body weight gain were not affected. These findings demonstrate that the volatile oil of N. sativa has the ability to inhibit colon carcinogenesis of rats in the postinitiation stage, with no evident adverse side effects, and that the inhibition may be associated, in part, with suppression of cell proliferation in the colonic mucosa.

PMID: 12881014 [PubMed - in process]

More Info – Clinical Studies – References – Buy Black Seed Oil now

Nigella sativa (black cumin) ameliorates potassium bromate-induced early events of carcinogenesis: diminution of oxidative stress.

Khan N, Sharma S, Sultana S.

Section of Chemoprevention and Nutrition Toxicology, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard, Hamdard University, New Delhi 110 062, India.

Potassium bromate (KBrO3) is a potent nephrotoxic agent. In this paper, we report the chemopreventive effect of Nigella sativa (black cumin) on KBrO3-mediated renal oxidative stress, toxicity and tumor promotion response in rats. KBrO3 (125 mg/kg body weight, intraperitoneally) enhances lipid peroxidation, gamma-glutamyl transpeptidase, hydrogen peroxide and xanthine oxidase with reduction in the activities of renal antioxidant enzymes and renal glutathione content. A marked increase in blood urea nitrogen and serum creatinine has also been observed. KBrO3 treatment also enhances ornithine decarboxylase (ODC) activity and [3H] thymidine incorporation into renal DNA. Prophylaxis of rats orally with Nigella sativa extract (50 mg/kg body weight and 100 mg/kg body weight) resulted in a significant decrease in renal microsomal lipid peroxidation (P < 0.001), gamma-glutamyl transpeptidase (P < 0.001), H2O2 (P < 0.001) and xanthine oxidase (P < 0.05). There was significant recovery of renal glutathione content (P < 0.01) and antioxidant enzymes (P < 0.001). There was also reversal in the enhancement of blood urea nitrogen, serum creatinine, renal ODC activity and DNA synthesis (P < 0.001). Data suggest that Nigella sativa is a potent chemopreventive agent and may suppress KBrO3-mediated renal oxidative stress, toxicity and tumour promotion response in rats.

PMID: 12755470 [PubMed - indexed for MEDLINE]

More Info – Clinical Studies – References – Buy Black Seed Oil now

Delayed apoptosis upon the treatment of Hep-2 cells with black seed.

Corder C, Benghuzzi H, Tucci M, Cason Z.

University of Mississippi Medical Center, Jackson, MS 39216, USA.

Nigella sativa (Black seed, BS) has been used to promote health and fight disease for centuries. The objectives of this investigation were: (1) to study whether agents such as cortisol and LPS alone or in combination induce cellular (Hep-2, laryngeal carcinoma) damage with time in culture (24, 48, and 72 hours) using apoptosis as a marker, (2) to determine if an immune stimulant such as BS, can protect Hep-2 cells from insult and ultimately thwart the programmed cells death mechanism. A total of 54 Hep-2 cell/tubes (50,000 cells per tube) were divided into six equal groups. Group one served as untreated control, while groups 2-6 were treated with either cortisol (10 ng/ml), LPS (10 micrograms/ml), BS (25 micrograms/ml), or a combination of LPS and cortisol and cortisol plus LPS plus BS, respectively. At the end of each phase the cells were harvested, heat fixed and stained with H&E to evaluate morphological changes. Immunohistochemistry, using antibodies against caspace-3 to evaluate cells undergoing apoptosis was conducted in all groups. The results of this study showed evidence of cells undergoing apoptosis at different magnitudes in all groups. However, the most dramatic change was seen in groups containing cortisol and LPS alone or in combination. This was supported by the fact that there were several adaptive responses observed in all phases. In addition, the exposure of BS to cells pretreated with cortisol and LPS showed evidence of protection against the progressive apoptosis.

PMID: 12724921 [PubMed - indexed for MEDLINE]

More Info – Clinical Studies – References – Buy Black Seed Oil now

Effect of Nigella sativa (N. sativa L.) and oxidative stress on the survival pattern of MCF-7 breast cancer cells.

Farah IO, Begum RA.

Jackson State University, Jackson, MS 39217, USA.

N. Sativa L., an oriental spice, has long been used as a natural medicine for treatment of many acute as well as chronic conditions. It has been used in the treatment of diabetes, hypertension, and dermatological conditions. There has been very few studies on the effects of N. Sativa as cancer prevention/therapy. Our objective therefore, was to expose MCF-7 breast cancer cells to aqueous and alcohol extracts and in combination with H2O2 as an oxidative stressor. Measurement of cell survival under various concentrations and combinations was conducted using standard cell culture techniques, exposure protocols in 96 well plates and Fluoro-spectrosphotometry. Following cellular growth to 90% confluency, exposure to water (WE) and ethanol (AE) extracts of N. sativa and H2O2 was performed. Toxicity index (LC50) was calculated from percent survival using regression analysis. Results showed that the alcohol extract and its combinations were able to completely inactivate the MCF-7 cells (LC50 ranged from 377.16-573.79 in descending potency for H2O2 + AE, AE and Mix of WE and AE). H2O2 alone effectively inactivated MCF-7 cells (LC50 = 460.94). The least effective combinations in descending potency were WE + H2O2, WE + AE + H2O2, and WE (LC50 were 725.79, 765.94, and 940.5 respectively. Combinations other than AE + H2O2 showed possible interactions, which lead to reduction in their potency. In conclusion, N. Sativa alone or in combination with oxidative stress were found to be effective in vitro in inactivating MCF-7 breast cancer cells, unveiling opportunities for promising results in the field of prevention and treatment of cancer.

PMID: 12724920 [PubMed - indexed for MEDLINE]


Protective effect of Nigella sativa seeds against carbon tetrachloride-induced liver damage.

Al-Ghamdi MS.

Department of Pharmacology, College of Medicine, King Faisal University, Dammam, Saudi Arabia. mghamdi@Dammam.kfu.edu.sa

It has been reported that Nigella sativa oil possesses hepatoprotective effects in some models of liver toxicity. However, it is N. sativa seeds that are used in the treatment of liver ailments in folk medicine rather than its oil. Therefore, the aim of this study was to investigate the effect of the aqueous suspension of N. sativa on carbon tetrachloride (CCL4)-induced liver damage. Aqueous suspension of the seeds was given orally at two dose levels (250 mg/kg and 500 mg/kg) for five days. CCL4 (250 microl/kg intraperitoneally / day in olive oil) was given to the experimental group on days 4 and 5, while the control group was only treated with the vehicles. Animals treated with CCL4 showed remarkable centrilobular fatty changes and moderate inflammatory infiltrate in the form of neutrophil and mononuclear cells when compared to the controls. This effect was significantly decreased in animals pretreated with N. sativa. Histopathological or biochemical changes were not evident following administration of N. sativa alone. Serum levels of aspartic transaminase (AST), and L-alanine aminotransferase (ALT) were slightly decreased while lactate dehydrogenase (LDH) was significantly increased in animals treated with CCL4 when compared to the control group. LDH was restored to normal but ALT and AST levels were increased in animals pretreated with N. sativa. In conclusion, N. sativa seeds appeared to be safe and possibly protective against CCL4-induced hepatotoxicity. However, further studies may still be needed prior to supporting its use in folk medicine for hepatic diseases.

PMID: 14696675 [PubMed - in process]

More Info – Clinical Studies – References – Buy Black Seed Oil now

Effect of Nigella sativa (black seed) on subjective feeling in patients with allergic diseases.

Kalus U, Pruss A, Bystron J, Jurecka M, Smekalova A, Lichius JJ, Kiesewetter H.

Humboldt University, Berlin, School of Medicine, Institute for Transfusion Medicine, Charite University Hospital, Berlin, Germany.

Nigella sativa (black seed) is an important medicinal herb. In many Arabian, Asian and African countries, black seed oil is used as a natural remedy for a wide range of diseases, including various allergies. The plant’s mechanism of action is still largely unknown. Due to the lack of study data on its efficacy in allergies, four studies on the clinical efficacy of Nigella sativa in allergic diseases are presented. In these studies, a total of 152 patients with allergic diseases (allergic rhinitis, bronchial asthma, atopic eczema) were treated with Nigella sativa oil, given in capsules at a dose of 40 to 80 mg/kg/day. The patients scored the subjective severity of target symptoms using a predefined scale. The following laboratory parameters were investigated: IgE, eosinophil count, endogenous cortisol in plasma and urine, ACTH, triglycerides, total cholesterol, LDL and HDL cholesterol and lymphocyte subpopulations. The score of subjective feeling decreased over the course of treatment with black seed oil in all four studies. A slight decrease in plasma triglycerides and a discrete increase in HDL cholesterol occurred while the lymphocyte subpopulations, endogenous cortisol levels and ACTH release remained unchanged. Black seed oil therefore proved to be an effective adjuvant for the treatment of allergic diseases. Copyright 2003 John Wiley & Sons, Ltd.

PMID: 14669258 [PubMed ]


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